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2005
Proceedings of the 6th Pacific Rim Conference on the Biotechnology of Bacillus thuringiensis and its Environmental Impact
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About the editors 
Page iii 
 
 
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Foreword from the editors 
Page iv 
 
 
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Mario Soberón, Isabel Gómez, Liliana Pardo, Carlos Muñoz, Luisa E. Fernandez, Claudia Pérez, Sarjeet S. Gill and Alejandra Bravo 
Important Interactions with Membrane Receptors in the Mode of Action of Bacillus thuringiensis Cry Toxins 
Pages 1-6 
 
 
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Liliana Pardo, Isabel Gómez, Carlos Muñoz-Garay, Nuria Jimenez-Juaréz, Jorge Sánchez, Claudia Pérez, Mario Soberón and Alexandra Bravo 
Oligomer Formation of Different Cry Toxins Indicates that a Pre-Poreis an Essential Intermediate in the Mode of Action of the Three-Domain Cry Family 
Pages 7-8 
 
 
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Claudia Pérez, Luisa E. Fernandez, Jianguang Sun, Jorge Luis Folch, Sarjeet S. Gill, Mario Soberón and Alejandra Bravo 
Cyt1Aa from Bacillus thuringiensis subsp. israelensis SynergizesCry11Aa Toxin Activity by Functioning as a Membrane-Bound Receptor 
Pages 9-11 
 
 
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Isabel Gómez, Juan Miranda-Ríos, Iván Arenas, Ricardo Grande, Baltazar Becerril, Alejandra Bravo and Mario Soberón 
Identification of scFv Molecules that Recognize Loop 3 of Domain IIand Domain III of Cry1Ab Toxin from Bacillus thuringiensis 
Pages 12-14 
 
 
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Yasuyuki Shitomi, Delwar M. Hossain, Kohsuke Haginoya, Masahiro Higuchi, Tohru Hayakawa, Kazuhisa Miyamoto, Ryoichi Sato and Hidetaka Hori 
Mechanism of Detoxification of Cry1Ac in Bombyx mori, Hybrid Shunreix Shogetsu 
Pages 15-16 
 
 
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Kazuya Tomimoto, Tohru Hayakawa and Hidetaka Hori 
Determination of a Region of Cry1Aa Inserted into Bombyx mori BBMV 
Pages 17-18 
 
 
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Liliane Meunier, Gabrielle Préfontaine, Manuela Van Munster, Roland Brousseau and Luke Masson 
Production and Characterization of a Subtractive cDNA Library andQuantitative PCR Analysis of Choristoneura fumiferana Genes Differentially Expressed inResponse to Bacillus thuringiensis Cry1Ab Toxin Exposure 
Pages 19-20 
 
 
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Manuela van Munster, Gabrielle Préfontaine, Alberto Mazza, Liliane Meunier, Miria Elias, Roland Brousseau and Luke Masson 
Gene Expression Response of the Spruce Budworm, Choristoneura fumiferana, after Exposure to Various Doses of Bacillus thuringiensis Cry1Ab Toxin Using Microarray Technology 
Pages 21-22 
 
 
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Sakae Kitada, Yuichi Abe, Akio Ito, Osamu Kuge, Tetsuyuki Akao, Eiichi Mizuki and Michio Ohba 
Molecular Identification and Cytocidal Action of Parasporin, a Protein Group of Novel Crystal Toxins Targeting Human Cancer Cells 
Pages 23-27 
 
 
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Luke Masson, Jarek Letowski, Alejandra Bravo and Roland Brousseau 
Using DNA Microarrays for Assessing Crystal Protein Genes in Bacillus thuringiensis 
Pages 28-30 
 
 
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Jen-Chieh Cheng, Feng-Chia Hsieh, Bing-Lan Liu and Suey-Sheng Kao 
Cloning and Expression of cry1Aa, cry1Ab, cry1C, and cry1Da Genesfrom Bacillus thuringiensis var. aizawai 
Pages 31-32 
 
 
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Xinyan Sylvia Liu and Donald H. Dean 
Redesigning Bacillus thuringiensis Cry1Aa Toxin into a Mosquito Toxin 
Pages 33-36 
 
 
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Vern Seligy, Gordon Coleman, Jennifer Crosthwait, Kathy Nguyen, Phil Shwed, Azam Tayabali, George Arvanitakis, Della Johnston, Louis Bryden, Michael Mulvey, Brian Belliveau and Esther Seto 
Development and Application of Molecular Tools for Exposure, Toxicityand Pathogenicity Characterization of Bacillus cereus Group Organisms in Context ofBiotechnology Use 
Pages 37-44 
 
 
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Imre S. Otvos, Holly Armstrong and Nicholas Conder 
Safety of Bacillus thuringiensis var. kurstaki Applications for Insect Control to Humans and Large Mammals 
Pages 45-60 
 
 
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David B. Levin 
Human Health Impact Assessment after Exposures to Bacillus thuringiensis subspecies kurstaki 
Pages 61-63 
 
 
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Mayumi Ohno, Miho Suganuma, Youko Egawa, Kazuya Tomimoto, Takashi Hara, Tohru Hayakawa and Hidetaka Hori 
A Comparative Study of Histamine Release from Rat Mast Cells byCry1Aa, Cry1Ab and Cry1Ac Fragmented with Simulated Gastric Fluid (SGF) 
Pages 64-66 
 
 
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Anthony M. Shelton, Jian-Zhou Zhao and Ping Wang 
Bt Resistance Management: Have We Been Lucky or Smart? 
Pages 67-71 
 
 
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Aiko Gryspeirt and Jean-Claude Grégoire 
A Deterministic Model to Evaluate and Improve the Strategy of InsectResistance Management (IRM) to Genetically Modified Plants Synthesizing a Cry Toxin 
Pages 72-73 
 
 
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Saad Mousa, Trilochan Mohapatra and Govind T. Gujar 
Monitoring of Bacillus thuringiensis Cry1Ac Resistance in Helicoverpa armigera (Hübner) (Noctuidae: Lepidoptera) 
Pages 74-77 
 
 
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Lisa Bird and Ray Akhurst 
Host Plant Effects Associated with Cry1A Resistance in Helicoverpa armigera 
Pages 78-80 
 
 
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Fernando H. Valicente, Newton P. Carneiro, Ruth H. Utida, Cláudia T. Guimarães, Jurandir V. Magalhães, Edilson Paiva and Andréa A. Carneiro 
Transformation of Maize Elite Lines with cry1Ca of Bacillusthuringiensis to Control Spodoptera frugiperda 
Pages 81-83 
 
 
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Mohsin Abbas Zaidi, Xiongying Cheng and Illimar Altosaar 
Bt Crop Straw is an Effective Source of Active and Stable Cry1Ac Toxin for Spray Bio-Formulations 
Pages 84-86 
 
 
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Mario Boisvert 
Utilization of Bacillus thuringiensis var. israelensis (Bti)-Based Formulations for the Biological Control of Mosquitoes in Canada 
Pages 87-93 
 
 
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Hyun G. Goh, Dale B. Gelman, Phyllis A. W. Martin, Ashaki D. S. Shropshire, Daniel L. Rowley, Michael B. Blackburn, Ik Y. Choi and Robert R. Farrar 
Insecticidal Effect of New Strains of Bacillus thuringiensis on theDiamondback Moth, Plutella xylostella 
Pages 94-96 
 
 
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Fernando H. Valicente, Rodrigo F. Zanasi, Kátia G. Boregas and Marliton R. Barretto 
Bacillus thuringiensis in Brazil: Geographical Distribution and Fermentation Media for Production 
Pages 97-98 
 
 
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Dirk Avé 
Valent BioSciences Corporation: an Overview 
Page 99 
 
 
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Paisan Ratanasatien, Uthai Ketunuti and Achara Tantichodok 
Positioning of Biopesticides in Thailand 
Pages 100-107 
 
 
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Takeshi Ito, Hisanori Bando and Shin-ichiro Asano 
Activation Process of the Mosquitocidal Delta-Endotoxin Cry39AaProduced by Bacillus thuringiensis subsp. aizawai BUN1-14 and Binding Property toAnopheles stephensi BBMV 
Pages 108-110 
 
 
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So Takebe, Shinji Morinaga, Akira Mizuhashi and Tohru Komano 
Improved Technique for Refining the Crystal of Bacillus thuringiensisby NaBr Gradient Centrifugation 
Pages 111-112 
 
 
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Yuichi Abe, Sakae Kitada, Osamu Kuge, Michio Ohba and Akio Ito 
Oligomerization of Parasporin-2, a New Crystal Protein fromNon-Insecticidal Bacillus thuringiensis, in Lipid Rafts 
Pages 113-115 
 
 
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Kohsuke Haginoya, Taisuke Kato, Yasuyuki Shitomi, Masahiro Higuchi, Ryoichi Sato, Tohru Hayakawa and Hidetaka Hori 
Kinetics of Interaction between Insecticidal Cry1A Toxins fromBacillus thuringiensis and Artificial Lipid Membrane Vesicles (Liposomes) 
Pages 116-117 
 
 
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Jong Yul Roh and Donald H. Dean 
Mutagenic Analysis of Putative Domain II and Surface Residues in Mosquitocidal Bacillus thuringiensis Cry19Aa Toxin 
Pages 118-121 
 
 
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Akiko Uemori, Minoru Maeda, Koichi Yasutake, Akira Ohgushi, Kumiko Kagoshima, Eiichi Mizuki and Michio Ohba 
Characterization of Cancer Cell-Killing Activity Associated withParasporal Proteins of Novel Bacillus thuringiensis Isolates 
Pages 122-123 
 
 
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Koichi Yasutake, Ngo Dinh Binh, Kumiko Kagoshima, Akiko Uemori, Akira Ohgushi, Minoru Maeda, Eiichi Mizuki, Yong Man Yu and Michio Ohba 
Occurrence of Bacillus thuringiensis Producing Parasporin, CancerCell-Killing Cry Proteins, in Vietnam 
Pages 124-125 
 
 
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Ngo Dinh Binh, Nguyen Xuan Canh, Nguyen Thi Anh Nguyet, Nguyen Dinh Tuan, Pham Kieu Thuy, Nguyen Thi Thanh Hanh, Shin-ichiro Asano and Michio Ohba 
Characterization of Bacillus thuringiensis Strains in the Vietnam Bacillus thuringiensis Collection 
Pages 126-130 
 
 
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Shin-ichiro Asano, Takuji Okamoto, Hisanori Bando, Mitsugu Horita, Hiroshi Sekiguchi and Toshihiko Iizuka 
Engineering Turf Grass for Resistance against Certain ColeopteranPests Using Bacillus thuringiensis cry8Da Gene 
Pages 131-132 
 
 
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Maureen O’Callaghan, E. Gerard and U. Sarathchandra 
Analysis of Non-Target Impacts of Foray 48B on Soil Micro-Organisms 
Pages 133-134 
 
 
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Valar Anoop, Wendy Shearer and Stuart Lee 
Ecosystem Effects of Novel Living Organisms (EENLO): A FederalResearch Initiative 
Page 135 
 
 
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Souad El Ouakfaoui, Lee-Ann Tsan, Théophile Paré and Kiera Delgaty 
Environmental Risk Assessment of Bacillus thuringiensis strain ATCC13367 under the Canadian Environmental Protection Act 1999 (CEPA 1999) 
Page 136 
 
 
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Lee Si-myung, Kim Jeoung-han, Park Byung-soo, Cho Hyun-suk, Kim Donghern and Jin Yong-moon 
Environmental Evaluation of GM Hot Pepper in Newly SynthesizedMaterial Differences 
Pages 137-138 
 
 
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Oliver G.G. Knox and Gupta V.S.R. Vadakattu 
Evaluation of Border Cell Number and Cry Protein Expression from Root Tips of Gossypium hirsutum 
Pages 139-140 
 
 
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Acknowledgements 
Page 141 
 
 
 
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